Spinach acyl-carrier proteins: structure, regulation, and progress towards cloning.
نویسندگان
چکیده
specificity is determined by the condensing enzyme, namely 8-ketoacyl-[acyl-carrier-proteinlsynthase 11. For the barley complex they are CoA derivatives which give rise to the C,,, C,, and C,, 3-oxoacyl key intermediates. One can speculate that the condensing enzyme activity specified by the cer-cqu-determined polypeptide is responsible for the noted substrate specificity. Therefore, should one wish to isolate the cer-cqu gene, whose product is part of either epidermal plasmalemma or cell walls, the most feasible approach is to exploit its expected homology with the gene for the condensing enzyme of palmitoyl elongase.
منابع مشابه
SPINACH ACYL CARRIER PROTEIN : PRIMARY STRUCTURE , mRNA TRANSLATION AND IMMUNOELECTROPHORETIC ANALYSIS 5501
INTRODUCTION Acyl carrier protein (ACP) is the central cofactor in plastid fatty acid synthesis (1) and in addition participates in desaturation (2) and acyl transfer reactions (3). In spinach mesophyll cells this protein is localized in the ch1orop1asts (4). In deve1opi ng soybean seeds the 1eve1s of acyl carri er protein are closely related to the rates of in Y..i.Y2. production of storage li...
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The initial step of fatty acid biosynthesis in both plants and Escherichia coli is the condensation of malonyl-acyl carrier protein with acetyl-COA, a reaction that is catalyzed by KAS 111 (Jackowski and Rock, 1987; Jackowski et al., 1989; Jaworski et al., 1989; Clough et al., 1992). In E. coli, KAS 111 has been implicated as a site of regulation for de novo fatty acid synthesis (Jackowski and ...
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Two forms of spinach acyl carrier protein (ACP-I and ACP-II) have recently been characterized and found to be expressed in a tissue-specific manner (JB Ohlrogge, TM Kuo, 1985 J Biol Chem 260: 8032). To examine possible different functions for these ACP isoforms, we have tested purified preparations of spinach leaf ACP-I and ACP-II and Escherichia coli ACP in several in vitro reactions of fatty ...
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Stearyl-acyl carrier protein desaturase (EC 1.14.99.6), present in the stroma fraction of spinach (Spinacia oleracea) chloroplasts, rapidly desaturated enzymatically prepared stearyl-acyl carrier protein to oleic acid. No other substrates were desaturated. In addition to stearyl-acyl carrier protein, reduced ferredoxin was an essential component of the system. The electron donor systems were ei...
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Stearyl acyl carrier protein, chemically prepared from 1-W stearic acid and Escherichia co2i acyl carrier protein, is desaturated to oleic acid by extracts of photoauxotrophic Euglena gracilis and of spinach. The soluble stearyl acyl carrier protein desaturase system from Eug~ena has been separated into three components, a reduced triphosphopyridine nucleotide oxidase, the desaturase, and a non...
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عنوان ژورنال:
- Biochemical Society transactions
دوره 14 3 شماره
صفحات -
تاریخ انتشار 1986